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OAU
Welcome to OAU, a standalone average nucleotide identity (ANI) calculator, which uses the improved ANI algorithm (OrthoANI) with USEARCH.

Overview

Although ANI is widely used to classify and identify bacteria, OrthoANI (Lee et al. 2015) was developed to overcome the large differences in reciprocal ANI values associated with the ANI algorithm. Furthermore, OAU employees USEARCH over BLAST for its OrthoANI calculations which increases the number of comparative studies and substantially decrease computational time.

Input

Genome FASTA files.

Output

Print results to stdout are in tabbed key-value pairs.

Citing this software

The manuscript is not yet available -- we will post a publication citation soon.

Requirements

  • Java Runtime Environment Version 8 (Java Download)
  • USEARCH (32-bit Download)
    *Although 64-bit version will reduce overall process time, we concluded that it is sufficient to use 32-bit version for a small number of genomes.
    Those of you who wish to compare large number of genomes, try our comparative genomics service here

Download OAU

  • OAU command line
OAU CMD JARDownload

Additional Resources

  • Example genome sets
Escherichia Coli SetDownload zip

License

OAU can be used without any restrictions. However, its redistribution in any way, form, shape, or derivative is strictly prohibited.

Support

If you experience any difficulties or have questions about OAU, please reach out to us at admin@ezbiocloud.net.

References

Yoon, S. H., Ha, S. M., Lim, J. M., Kwon, S.J. & Chun, J. (2017). A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek. [Full Text]