Welcome to CJ Bioscience's Orthologous Average Nucleotide Identity Tool (OAT), a similarity measurement tool for genomes.
Overview
OAT uses OrthoANI to measure the overall similarity between two genome sequences. ANI and OrthoANI are comparable algorithms: they share the same species demarcation cut-off at 95~96% and large comparison studies have demonstrated both algorithms to produce near identical reciprocal similarities. Details of the OrthoANI algorithm is given in (Lee et al. 2015). OAT employs an easy-to-follow Graphical User Interface that allow researchers to calculate OrthoANI values between genomes of interest without unfamiliar Command Line Environments. Moreover, the OAT_cmd command-line software can be integrated into preexisting bioinformatics pipelines.
Update Notes
(2015-10-26) GGDC calculator is updated (http://ggdc.dsmz.de/), calculating GGDC1, GGDC2, and GGDC3.
(2017-06-14) User can now specify the number of worker threads.
Input
Genome FASTA files.
Output
CSV file.
Citing this software
Lee, I., Kim, Y. O., Park, S. C., & Chun, J. (2015). OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol. 66: 1100-1103.
[Full Text]
NCBI BLAST is required if you are using the Runnable JAR version of OAT.
We recommend ncbi-blast-2.2.30+ or higher since OAT was tested with ncbi-blast-2.2.30+ (BLAST+ executables)
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