OAT uses OrthoANI to measure the overall similarity between two genome sequences. ANI and OrthoANI are comparable algorithms: they share the same species demarcation cut-off at 95~96% and large comparison studies have demonstrated both algorithms to produce near identical reciprocal similarities. Details of the OrthoANI algorithm is given in (Lee et al. 2015). OAT employs an easy-to-follow Graphical User Interface that allow researchers to calculate OrthoANI values between genomes of interest without unfamiliar Command Line Environments. Moreover, the OAT_cmd command-line software can be integrated into preexisting bioinformatics pipelines.

Update Notes
(2015-10-26) GGDC calculator is updated (http://ggdc.dsmz.de/), calculating GGDC1, GGDC2, and GGDC3.
(2017-06-14) User can now specify the number of worker threads.

Genome FASTA files.

CSV file.

Lee, I., Kim, Y. O., Park, S. C., & Chun, J. (2015). OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol. 66: 1100-1103. [Full Text]

• Java Runtime Environment Version 8 (Java Download)
• NCBI BLAST is required if you are using the Runnable JAR version of OAT. We recommend ncbi-blast-2.2.30+ or higher since OAT was tested with ncbi-blast-2.2.30+ (BLAST+ executables)

• OAT standalone

• OAT command line

• Example Genome Sets

OAT can be used without any restriction. However, its redistribution in any way, form, shape, or derivative is strictly prohibited.

If you experience any difficulties or have questions about the OAT application, please reach out to us at lebmaster.snu+oat.help@gmail.com

	Yeong Ouk Kim Yeong Ouk Kim received his B.S. in Computer Science and Applied Statistics at Purdue University (2012) and is currently a MS-Ph.D candidate in Bioinformatics at Seoul National University.
	Imchang Lee Imchang Lee is currently a MS-Ph.D candidate in Bioinformatics at Seoul National University.
	Sang-Cheol Park Sang-Cheol Park received his B.S. at Seoul National University and is currently a MS-Ph.D candidate in Bioinformatics at Seoul National University.
	Jongsik Chun Jongsik Chun is a professor at Seoul National University and specializes in microbial evolution, bioinformatics, and genomics. He has been a trustee of Bergey’s Manual Trust since 2010 and is also the founder of ChunLab, Inc.