Welcome to OrthoANIu tool, a standalone average nucleotide identity (ANI) calculator, which uses the improved ANI algorithm (OrthoANI) with USEARCH.
Although ANI is widely used to classify and identify bacteria,
OrthoANI (Lee et al. 2015)
was developed to overcome the large differences in reciprocal ANI values associated with the ANI algorithm. Furthermore, OrthoANIu tool
employees USEARCH over BLAST for its OrthoANI calculations which increases the number of comparative studies and substantially decrease
computational time. [Learn more]
Genome FASTA files.
Print results to stdout are in tabbed key-value pairs.
Citing this software
Yoon, S. H., Ha, S. M., Lim, J. M., Kwon, S.J. & Chun, J. (2017). A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek. 110:1281–1286.[Full Text]
USEARCH (32-bit Download)
*Although 64-bit version will reduce overall process time,
we concluded that it is sufficient to use 32-bit version
for a small number of genomes.
Those of you who wish to compare large number of genomes, try our comparative genomics service
Users affiliated with academic and non-profit institutions are entailed to
free use of EzBioCloud's database and bioinformatics applications with conditions. Reproduction or redistribution of EzBioCloud is strictly prohibited by
applicable law and regulations.
For the following cases, please contact firstname.lastname@example.org to obtain a license to
use our services.
Use in for-profit institutions or companies.
Use for commercial projects by academic or non-profit organizations
Please login to the site to download the database.
Click here to
login or register if you do not have an account
Your institution doesn't appear to be registered with us. Please request
institution verification by clicking the button below.
The email domain associated with your EzBioCloud account doesn't
appear to be an institution that we recognize. Please do not use gmail, hotmail
etc. Use your institutional email.
If you believe this is an error, please contact email@example.com for further
By not agreeing to only use EzBioCloud for non-profit purposes, you will not be granted free access to our database and bioinformatics applications on EzBioCloud.
Please Confirm or Go back to change your status.
This browser is not supported.
We recommend to download the following browser from the link below.