Welcome to OrthoANIu tool, a standalone average nucleotide identity (ANI) calculator, which uses the improved ANI algorithm (OrthoANI) with USEARCH.
Although ANI is widely used to classify and identify bacteria,
OrthoANI (Lee et al. 2015)
was developed to overcome the large differences in reciprocal ANI values associated with the ANI algorithm. Furthermore, OrthoANIu tool
employees USEARCH over BLAST for its OrthoANI calculations which increases the number of comparative studies and substantially decrease
computational time. [Learn more]
Genome FASTA files.
Print results to stdout are in tabbed key-value pairs.
Citing this software
Yoon, S. H., Ha, S. M., Lim, J. M., Kwon, S.J. & Chun, J. (2017). A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek. 110:1281–1286.[Full Text]
USEARCH (32-bit Download)
*Although 64-bit version will reduce overall process time,
we concluded that it is sufficient to use 32-bit version
for a small number of genomes.
Those of you who wish to compare large number of genomes, try our comparative genomics service